SynBio Read/Write/Edit Homework Assignment

<aside> ⚠️ About your Documentation Make sure to document every step of the in-silico and lab experiments. Make sketches, screenshots, notes, drawings - anything that helps you - and others understand the experiment. Your documentation should help you - and others - to understand the topic. Don’t be afraid to add things that don’t work. Show your failures - and how you did overcome them. Your Documentation should be a description of the amazing journey you are on!

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Part 0: Basics of Gel Electrophoresis

• [x] Watch all lecture and recitation videos.
  • [ ] Optionally watch bootcamp

Part 1: Benchling & In-silico Gel Art

See the Gel Art: Restriction Digests and Gel Electrophoresis protocol for details. Overview:

• [x] Make a free account at benchling.com
• [x] Import the Lambda DNA.

• [x] Simulate Restriction Enzyme Digestion with the following Enzymes: EcoRI HindIII BamHI KpnI EcoRV SacI SalI

EcoRI Restriction Enzyme Digestion Simulation

HinDIII Restriction Enzyme Digestion Simulation

BamHI Restriction Enzyme Digestion Simulation

KpnI Restriction Enzyme Digestion Simulation

SacI Restriction Enzyme Digestion Simulation

SalI Restriction Enzyme Digestion Simulation

EcoRI, HindIII, BamHI, KpnI, EcoRV, SacI, SalI Simultaneous Simulation Enzyme Digest

EcoRI, HindIII, BamHI, KpnI, EcoRV, SacI, SalI Summarised Simulation Enzyme Digest

Part 2: Gel Art - Restriction Digests and Gel Electrophoresis